DNA-Gel-Stain
DNA-Gel-Stain

DeNA Gel Stain

Product Code: DNT-GS
The DeNA Gel Stain from Denagene Tajhiz is an advanced, non-mutagenic fluorescent gel stain designed to provide safe, precise, and high-resolution visualization of DNA and RNA in agarose gels. In modern molecular biology, genetics, and biotechnology laboratories, researchers require reagents that are not only effective but also safe for frequent use. Traditional ethidium bromide, while effective, poses mutagenic risks, limiting its use in educational and clinical laboratories. DeNA Gel Stain addresses this concern by offering a validated non-mutagenic alternative, confirmed by the Ames test, allowing laboratories to maintain safety standards without compromising on sensitivity or accuracy.
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Overview of Laboratory DeNA Gel Stain

The DeNA Gel Stain from Denagne Tajhiz is a state-of-the-art fluorescent gel stain specifically developed to meet the rigorous demands of modern molecular biology, genetics, and biotechnology laboratories. Unlike traditional DNA stains such as ethidium bromide, which carry mutagenic and health hazards, DeNA Gel Stain has been validated by the Ames test to ensure it is non-mutagenic, making it a safe alternative for daily laboratory use. This safety profile is particularly important in clinical, educational, and high-throughput research settings where repeated exposure to mutagenic chemicals could pose long-term risks to laboratory personnel.

One of the key advantages of DeNA Gel Stain is its extreme efficiency and low required volume. With a concentration of 20000X, only 1 µL per 20 mL of molten agarose gel is sufficient for effective staining. This allows researchers to conserve reagents, reduce costs, and maintain consistency across multiple experiments. The stain is capable of binding to double-stranded DNA, single-stranded DNA, and RNA, providing broad applicability for a variety of experiments, from PCR product visualization to RNA integrity assessment. Its high fluorescence intensity ensures bright, sharp, and well-defined bands, which simplifies quantification, documentation, and interpretation of results.

Another notable feature of DeNA Gel Stain is its universal compatibility with all UV and SAFE transilluminators. Whether a laboratory uses traditional UV gel documentation systems or newer blue-light SAFE systems, this stain delivers consistent, reproducible results without requiring specialized equipment. Its versatility extends to SDS-PAGE protein gels, enabling biochemical labs to use the same reagent for DNA, RNA, and protein applications, streamlining workflows and reducing the need for multiple staining reagents.

Designed with ease of use and reliability in mind, DeNA Gel Stain is simple to integrate into routine laboratory procedures. The addition of 1 µL per 20 mL of molten gel ensures uniform distribution, minimizing human error and allowing even novice users to achieve high-quality results. Its long-term stability at 4°C for up to one year ensures that laboratories can store and use the reagent over extended periods without degradation in performance.

In summary, the DeNA Gel Stain is a highly effective, safe, and versatile gel stain that balances laboratory safety, cost-efficiency, and superior performance. Its precise staining capabilities, broad compatibility, and robust formulation make it an indispensable tool for molecular biology, genetics, biotechnology, and clinical research laboratories that demand accurate and reproducible visualization of DNA and RNA.

Key Features of DNA Gel Stains

The DeNA Gel Stain by Denagene Tajhiz offers a wide range of features that make it an indispensable reagent for molecular biology, genetics, and biotechnology laboratories. One of the most important aspects of this stain is its high sensitivity and accuracy. It efficiently binds to double-stranded DNA, single-stranded DNA, and RNA, providing researchers with clear, sharp, and easily distinguishable bands. This level of precision is crucial for detecting low-abundance nucleic acids, verifying PCR products, or assessing the quality and integrity of RNA samples. High sensitivity ensures that even faint DNA or RNA fragments are visible, improving the reliability of experimental results and downstream analyses.

Safety is another defining feature of DeNA Gel Stain. Traditional DNA stains, such as ethidium bromide, pose mutagenic risks, which limit their use in teaching laboratories, clinical environments, and high-throughput research. DeNA Gel Stain addresses this problem with a non-mutagenic formulation validated by the Ames test, making it safe for routine use. Laboratory personnel can handle the reagent without extensive protective measures, and students in educational labs can perform DNA visualization experiments with minimal risk.

Ease of use is a further advantage. The stain is extremely concentrated (20000X), meaning only 1 µL per 20 mL of molten agarose gel is required for effective staining. This simplifies preparation, reduces reagent waste, and ensures consistency across multiple gels. Researchers can achieve uniform staining without complicated protocols, saving time and minimizing potential for human error.

In addition, the stain exhibits high fluorescence intensity, producing bright, well-defined bands that are easily captured by both UV and SAFE transilluminators. Its versatility allows it to be used not only for DNA and RNA visualization but also for SDS-PAGE protein gels, extending its utility to biochemical applications. Laboratories benefit from a durable product with a shelf life of up to one year when stored at 4°C, ensuring long-term usability without loss of performance.

Other notable features include compatibility with a wide range of transilluminators, cost-effectiveness due to minimal usage per gel, and reproducibility of results across different experimental setups. In essence, the DeNA Gel Stain combines safety, sensitivity, versatility, and convenience, making it a reliable choice for research, clinical, and educational laboratories seeking high-quality DNA and RNA visualization.

Applications of Agarose Gel Stain

The DeNA Gel Stain by Denagene Tajhiz is a versatile and highly reliable reagent, designed to meet the diverse needs of molecular biology, genetic research, biotechnology, and clinical laboratories. One of its primary applications is the visualization and analysis of DNA and RNA on agarose gels. By binding selectively to nucleic acids and fluorescing under UV or SAFE light, it allows researchers to detect DNA fragments of various sizes, assess the integrity of RNA samples, and quantify nucleic acid concentrations. This capability is essential for experiments such as PCR product analysis, restriction fragment analysis, and genomic DNA quality assessment, where accurate visualization directly influences downstream experimental success.

Another critical application is the assessment of PCR products. During PCR amplification, confirming the presence, size, and purity of amplified DNA is essential before proceeding to cloning, sequencing, or further genetic analysis. DeNA Gel Stain provides bright, clear bands, enabling researchers to quickly verify the success of amplification, identify unexpected fragments, and avoid costly mistakes. Its high sensitivity ensures that even low-abundance PCR products are easily detected, improving reproducibility and confidence in experimental results.

The stain is also valuable in research related to molecular biology, genetics, and biotechnology, where precise DNA and RNA visualization is a routine requirement. It supports studies involving gene expression analysis, plasmid verification, RNA integrity checks, and genetic engineering experiments. In addition, its non-mutagenic and safe formulation makes it suitable for use in educational laboratories, where students can perform nucleic acid staining experiments without exposure to hazardous chemicals.

DeNA Gel Stain can also be employed in diagnostic and clinical laboratories for DNA/RNA analysis in patient samples. Accurate visualization of nucleic acids is crucial for identifying genetic disorders, analyzing viral loads, or assessing the integrity of RNA in clinical specimens. Furthermore, its application extends beyond nucleic acids; it can be used for protein gel staining in SDS-PAGE, allowing laboratories to standardize reagents for multiple experimental purposes, streamline workflows, and reduce the need for separate staining solutions.

By providing a combination of safety, efficiency, and high-quality visualization, DeNA Gel Stain ensures reproducible and accurate results across a broad spectrum of laboratory applications. Its ease of use, compatibility with all transilluminators, and versatility make it an essential tool for research, clinical diagnostics, and educational settings, offering reliable performance for every nucleic acid or protein staining requirement.

Advantages of DNA Gel Stain

The DeNA Gel Stain from Denagene Tajhiz offers a wide array of advantages that make it a superior choice for molecular biology, genetics, biotechnology, and clinical laboratories. One of the primary benefits is enhanced safety. Unlike traditional stains such as ethidium bromide, which are mutagenic and require strict handling protocols, DeNA Gel Stain is non-mutagenic and validated by the Ames test, ensuring a safer laboratory environment. This significantly reduces the health risks to laboratory personnel and allows for its use in educational settings where students can handle reagents without extensive protective measures. Safety, therefore, becomes a cornerstone of laboratory workflow, particularly in high-throughput or teaching laboratories.

Another significant advantage is high clarity and precision in DNA and RNA visualization.Agarose Gel Stain produces bright, sharp, and well-defined bands, allowing researchers to easily identify and quantify nucleic acid fragments. This is particularly important for applications such as PCR product verification, restriction analysis, and RNA integrity assessment. High clarity ensures that even low-abundance DNA fragments are detectable, improving the accuracy and reproducibility of experimental results. Accurate visualization is also critical when samples are prepared for downstream applications like cloning, sequencing, or genetic engineering, reducing the chances of errors or misinterpretation.

Cost-effectiveness is another notable advantage. The Gel Stain is extremely concentrated (20000X), requiring only 1 µL per 20 mL of agarose gel, which minimizes reagent usage and reduces operational costs over time. This efficiency makes it suitable for laboratories performing large-scale experiments without compromising on staining quality. Additionally, the product offers long-term stability, maintaining its staining efficiency for up to one year when stored at 4°C, ensuring reliability across multiple experiments and consistent performance over time.

Ease of use is also a key benefit. The stain’s simple workflow—adding a minimal amount to molten agarose, mixing, casting, and running electrophoresis—reduces the risk of preparation errors and saves valuable laboratory time. Its compatibility with all UV and SAFE transilluminators ensures flexibility across different laboratory setups. Furthermore, its versatility extends to SDS-PAGE protein gels, allowing a single reagent to be used for multiple experimental purposes.

In summary, the DNA Gel Stain combines safety, precision, cost-effectiveness, ease of use, long-term stability, and versatility, making it a highly valuable tool for research, diagnostic, and educational laboratories. It enhances workflow efficiency, ensures reliable visualization, and provides peace of mind for laboratory professionals handling sensitive nucleic acid samples.

How to Use DeNA Gel Stain

Using the DeNA Gel Stain from Denagene Tajhiz is designed to be straightforward, efficient, and safe, ensuring that laboratory personnel can achieve consistent, high-quality visualization of DNA and RNA bands without unnecessary complexity. The first step involves preparing the agarose gel as usual. Once the gel has melted and reached an appropriate temperature for pouring, the DeNA Gel Stain is added directly to the molten agarose. The recommended concentration is 1 µL of DeNA Gel Stain per 20 mL of agarose gel, which provides optimal staining while conserving reagent and maintaining cost-effectiveness. This precise dilution ensures uniform distribution of the fluorescent molecules throughout the gel matrix.

After adding the stain, the gel must be thoroughly mixed to guarantee even incorporation. Careful mixing prevents uneven staining, which could lead to inaccurate visualization of DNA or RNA bands. Once the stain is evenly distributed, the agarose is poured into the gel casting tray and allowed to solidify completely. During this process, standard laboratory precautions should be followed to avoid contamination and ensure proper gel formation. The solidified gel is then ready for electrophoresis, where samples containing DNA or RNA are loaded into wells and subjected to an electric field.

Following electrophoresis, visualization of the nucleic acids is achieved using any UV or SAFE transilluminator. The DeNA Gel Stain fluoresces under UV or safe-light conditions, producing bright, well-defined bands that facilitate both qualitative and quantitative analysis. The high sensitivity of the stain allows detection of even low-concentration samples, making it suitable for PCR product verification, DNA quality assessment,

RNA integrity checks, and other routine molecular biology applications.
For laboratories that also perform SDS-PAGE protein gel analysis, DeNA Gel Stain can be applied as an alternative staining method, further extending its versatility. After imaging, gels can be safely disposed of following laboratory waste guidelines, and the small volume of stain used ensures minimal environmental impact.

The straightforward workflow, combined with the non-mutagenic and stable formulation of DeNA Gel Stain, guarantees reproducible results, minimizes errors, and enhances laboratory efficiency. By integrating safety, ease of use, and high-quality fluorescence, DeNA Gel Stain provides researchers with a reliable tool for accurate DNA and RNA visualization in a wide range of molecular biology, biotechnology, and clinical laboratory applications.

Denagene Tajhiz DeNA Gel Stain

The Denagene Tajhiz DeNA Gel Stain represents a professional-grade solution for laboratories seeking safe, efficient, and reliable DNA and RNA visualization. Developed by Denagene Tajhiz, a leading supplier of high-quality laboratory reagents and equipment, this gel stain combines advanced formulation with user-friendly handling, making it an indispensable tool for molecular biology, genetics, biotechnology, and clinical research laboratories. Its non-mutagenic properties, validated by the Ames test, ensure that laboratory personnel can work safely without exposure to the health risks typically associated with traditional ethidium bromide stains. This safety feature is especially valuable in educational and teaching labs, where students can conduct experiments with minimal risk while still obtaining precise results.

The DeNA Gel Stain is extremely concentrated (20000X), meaning only 1 µL per 20 mL of molten agarose gel is required for effective staining. This efficiency not only reduces reagent usage but also lowers operational costs over time, making it a cost-effective choice for laboratories with high-throughput workflows. Its versatility allows for the visualization of double-stranded DNA, single-stranded DNA, and RNA, ensuring that researchers can reliably assess a variety of nucleic acid samples. Furthermore, the stain is fully compatible with all UV and SAFE transilluminators, providing flexibility for laboratories with different imaging systems and ensuring consistent, high-quality fluorescence for both routine and advanced experiments.

In addition to nucleic acid applications, the DeNA Gel Stain can also be used for SDS-PAGE protein gel visualization, extending its functionality beyond DNA and RNA analysis. This multi-purpose use makes it an excellent choice for labs seeking to streamline their workflow with a single, reliable reagent. The stain is stable for up to one year when stored at 4°C, maintaining its high fluorescence efficiency and reproducibility throughout its shelf life.

Laboratories using the Denagene Tajhiz DeNA Gel Stain benefit from a combination of safety, precision, versatility, and ease of use, making it suitable for research, diagnostic, and educational purposes. Researchers can confidently detect and quantify nucleic acids, prepare samples for downstream applications, and document results efficiently.

 

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    FAQ

    1.. What is the primary purpose of DeNA Gel Stain?

    It is a non-mutagenic fluorescent stain for safe and precise visualization of DNA and RNA on agarose gels.

    2. Is it compatible with UV and SAFE transilluminators?

    Yes, it is fully compatible with all UV and SAFE transilluminators.

    3. How much stain is required per gel?

    Only 1 µL per 20 mL of molten agarose gel is sufficient for clear staining.
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