Electrophoresis, a crucial technique in laboratories, utilizes electricity to separate molecules such as proteins or nucleic acids based on their size, structure, and electrical charge. Electrophoresis units, also referred to as electrophoresis chambers or electrophoresis systems, are devices specifically designed for performing this separation. These units consist of a gel tray (or capillary array), a buffer tank, and a power supply. Additionally, many units incorporate safety features, control panels, and integrated imaging systems to prevent electric shocks and facilitate analysis. Regardless of their specific type, electrophoresis units serve as versatile tools for visualization and analysis, necessitating regular care and maintenance.
When choosing an appropriate place for your electrophoresis unit, it is vital to consider several significant factors. First and foremost, prioritize safety by keeping the device away from sinks or any other water sources to prevent potential electrical hazards. Secondly, make sure that the electrophoresis unit and its power supply are positioned in a way that allows easy access to the on/off switch and provides clear visibility of the power indicator light. Lastly, arrange the tool in a manner that maintains tidy organization of the leads, preventing them from dangling. For added convenience and to protect your workspace, consider placing a layer of water-absorbent benchtop paper to catch any buffer drips and spills that may occur, making the unit feel more at home.
To ensure successful and safe experiments, it is crucial to perform an annual inspection of your electrophoresis equipment. The following steps, which typically take between 10 and 30 minutes (depending on the number of machines), will save you time in the long term. If you have backup electrophoresis units, it is advisable to conduct this check just before your first experiment, as everything will already be set up. If not, we recommend performing the inspection before the term begins, allowing ample time for any necessary repairs or replacements.
1-Begin by turning off the unit and unplugging it.
2-Inspect the power cords for any signs of deterioration, such as exposed wires, cracks, or breaks.
3-Examine the connectors for corrosion or loose fittings, as these can cause electrical arcing and potential fire hazards. Also, check for any exposed copper wires resulting from improper removal of the jack (pulling on the wire instead of the jack).
4-Thoroughly examine the electrophoresis chamber for any cracks in the plastic that could lead to leaks.
5-For vertical (protein) electrophoresis chambers, also verify that the gaskets are not leaking.
6-Dispose of and replace any cords, connectors, chambers, or gaskets that fail the inspection.
7-(Optional) Confirm the full functionality of the unit by adding a buffer, powering it on, and observing. Within 5-10 minutes, you should observe bubbles in the buffer, indicating proper functioning.
Let's start by prioritizing safety before delving into the fascinating realm of cleaning your electrophoresis unit. Remember to always disconnect the cord to avoid any surprising shocks. Now, the cleaning methods for your specific unit may differ, so it's important to verify the instructions provided by the manufacturer.
Begin by cleaning the chamber, gel beds, and combs. For these components, simply wash them with tap water or distilled/deionized water and allow them to dry naturally. There's no need for detergents in this case! Since this equipment falls into the category of "sensitive skin" gear, it's best to avoid using harsh cleansers containing organic solvents, acids, or alkalis. Moving on to the power sources, they don't require extensive cleaning, but they still deserve some attention. Disconnect them and wipe them down with a dry cloth.
Units equipped with integrated visualization require a slightly different cleaning approach. To clean these, use a mildly damp, soapy cloth for wiping. Be cautious not to allow water to seep into the unit. Avoid using abrasive cleaners or strong solvents. If necessary, you can fully submerge the buffer chamber, tray, combs, and endcaps in mild detergent for cleaning purposes.
Only utilize appropriate electrophoresis buffers in the chamber. Improper buffers not only risk damaging the power supply and electrodes but also pose an electric shock hazard to you.
Prioritize safety
Remember to deactivate the power before opening the lid or reaching inside the chamber. We wouldn't want any unexpected sparks or surprises, right? Always power down before engaging.
Since electrophoresis units deal with high voltages, it's best to entrust any disassembly or servicing tasks to properly trained professionals. We want everyone to remain safe while maintaining these units. Similarly, resist the temptation to modify or bypass the safety features of your unit. Safety measures exist for a reason—to keep you and your equipment protected.
For electrophoresis units equipped with integrated visualization systems, remember not to submerge the outer housing or pour liquids onto the unit. Water may quench our thirst, but it won't benefit our units. Keep them dry and content!
When working with electrophoresis units featuring integrated visualization systems, it's important to wear suitable eyewear. While some units use blue light, which may not necessarily require specialized safety glasses, it's still high intensity, so avoid prolonged staring without an orange lid in place.
Whenever working with DNA stains, including "safer" non-ethidium bromide stains, ensure that you wear gloves and follow all manufacturer recommendations. Safety should never be neglected, so handle chemicals responsibly and always utilize personal protective equipment.